Portable, low cost and quantitative nucleic acid detection is desirable for point-of-care diagnostics. However, current standards such as PCR often require multiple sample preparation steps and expensive non-portable equipment. mFluiDx is developing low-cost, multiplexed, simple, rapid and portable microfluidic diagnostic assays for point-of-care applications. Our diagnostic platform paves the way for a new genre of diagnostics--assays with the simplicity of dipstick tests (i.e. pregnancy tests), while providing multiplexed (or quantitative) nucleic acid amplification detection capability.